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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
29/10/1998 |
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Data da última atualização: |
15/01/2014 |
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Autoria: |
NICODEMO, M. L. F. |
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Afiliação: |
EMBRAPA. Centro Nacional de Pesquisa de Gado de Corte (Campo Grande, MS). |
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Título: |
Measurement and function of turnover markers in sheep and pig bone. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
[S.l.] : University of Aberdeen, 1997. |
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Páginas: |
204p. |
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Idioma: |
Inglês |
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Notas: |
Tese Doutorado. CNPGC. |
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Conteúdo: |
Plasma osteocalcin and the urinary pyridinium crosslinks, pyridinoline and deoxypyridinoline are widely used as markers of bone tumover though many aspects of their function and metabolism remain obscure. In this thesis several likely physiological determinants of the levels of these markers in blood, bone and urine were examined in sheep and in pigs. Measurement of osteocalcin proved a major problem, immunoassay being unreliable as a method for measuring the levels of this protein in bone. This necessitated the development of an RP-HPLC assay (Chapter 3) in which the protein was separated from bone extracts using a linear 4-60% acetonitrile gradient containing 0. I % TFA run over 45 mins at a flow rate of lml/min. The standard curve was linear over a range of 0.5-15.0 pg osteocalcin injected and with inter- and intra-assay coefficients of variation of 6.9 and 8.8 §lo respectively and with recovery of osteocalcin added to bone extracts averaging 102.7+ 6,16 %. In sheep, plasma osteocalcin levels, determined by ELISA, showed large between animal as well as variability over 24 hours but no evidence of consistent circadian rhythm and decreased with age. In bone, changes in osteocalcin level tended to parallel those for calcium, whereas levels of the pyridinium crosslinks tended to increase with age (Chapter 4). Neither group of markers were sufficiently sensitive to detect differences in bone tumover between lambs of similar age but growing at different rates (Chapter 5). At slaughter the faster growing lambs had heavier though less well mineralised bones but showed no change in the ratio of calcium/osteocalcin. The ratio of calcium/osteocalcin in bone was also unchanged in lambs fed diets deficient in phosphorus (Chapter 6) despite an overall decline in mineral and in plasma and bone osteocalcin contents, which appeared to support the view that plasma and bone osteocalcin contents, which appeared to support the view thatosteocalcin may play a functional role in the mineralization process. However, against this, pigs fed diets deficient in both calcium and phosphorus showed no change in either plasma or bone osteocalcin levels despite marked reductions in bone calcium content (Chapter 7). This suggests that the amount of osteocalcin deposited in bone is not simply related to the amount of mineral present and so calls into question whether it has a direct role in the mineralization process. Chapter 8 describes an experiment in which adult sheep were treated with one of the new bisphosphonates (Ibandronate) and its effects on the metabolism of both pyridinoline and deoxypyridinoline were monitored. Given at rates which have been shown to be effective in reducing bone resorption in humans, this compound had little effect on the overall rates of excretion of these crosslinks but did alter the proportions excreted in free or peptide bound form. Another feature of this study was the high variability seen in the escretion of these markers in urine both between animals and between collection periods with coeficients of variation of 48 and 36 % for total pyridinoline and deoxypyridinoline and 62 and 50 % for their free fractions. Similar high variability has been reported by other workers and has clear implications in regard to the accuracy of the these markers. MenosPlasma osteocalcin and the urinary pyridinium crosslinks, pyridinoline and deoxypyridinoline are widely used as markers of bone tumover though many aspects of their function and metabolism remain obscure. In this thesis several likely physiological determinants of the levels of these markers in blood, bone and urine were examined in sheep and in pigs. Measurement of osteocalcin proved a major problem, immunoassay being unreliable as a method for measuring the levels of this protein in bone. This necessitated the development of an RP-HPLC assay (Chapter 3) in which the protein was separated from bone extracts using a linear 4-60% acetonitrile gradient containing 0. I % TFA run over 45 mins at a flow rate of lml/min. The standard curve was linear over a range of 0.5-15.0 pg osteocalcin injected and with inter- and intra-assay coefficients of variation of 6.9 and 8.8 §lo respectively and with recovery of osteocalcin added to bone extracts averaging 102.7+ 6,16 %. In sheep, plasma osteocalcin levels, determined by ELISA, showed large between animal as well as variability over 24 hours but no evidence of consistent circadian rhythm and decreased with age. In bone, changes in osteocalcin level tended to parallel those for calcium, whereas levels of the pyridinium crosslinks tended to increase with age (Chapter 4). Neither group of markers were sufficiently sensitive to detect differences in bone tumover between lambs of similar age but growing at different rates (Chapter 5). At sl... Mostrar Tudo |
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Palavras-Chave: |
Biochemical markers; Bone markers; Deoxypiridinoline; Diagnosis; Dioxidipirionolina; Marcador bioquimico; Marcador osseo; Metabolismo osseo; Osteocalcina; Ovine; Piridinolina; Pyridinoline. |
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Thesagro: |
Diagnostico; Fisiologia; Osso; Ovino; Suíno. |
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Thesaurus Nal: |
bone metabolism; bones; osteocalcin; physiology; swine. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04307nam a2200397 a 4500
001 1319734
005 2014-01-15
008 1997 bl uuuu m 00u1 u #d
100 1 $aNICODEMO, M. L. F.
245 $aMeasurement and function of turnover markers in sheep and pig bone.
260 $a[S.l.] : University of Aberdeen$c1997
300 $a204p.
500 $aTese Doutorado. CNPGC.
520 $aPlasma osteocalcin and the urinary pyridinium crosslinks, pyridinoline and deoxypyridinoline are widely used as markers of bone tumover though many aspects of their function and metabolism remain obscure. In this thesis several likely physiological determinants of the levels of these markers in blood, bone and urine were examined in sheep and in pigs. Measurement of osteocalcin proved a major problem, immunoassay being unreliable as a method for measuring the levels of this protein in bone. This necessitated the development of an RP-HPLC assay (Chapter 3) in which the protein was separated from bone extracts using a linear 4-60% acetonitrile gradient containing 0. I % TFA run over 45 mins at a flow rate of lml/min. The standard curve was linear over a range of 0.5-15.0 pg osteocalcin injected and with inter- and intra-assay coefficients of variation of 6.9 and 8.8 §lo respectively and with recovery of osteocalcin added to bone extracts averaging 102.7+ 6,16 %. In sheep, plasma osteocalcin levels, determined by ELISA, showed large between animal as well as variability over 24 hours but no evidence of consistent circadian rhythm and decreased with age. In bone, changes in osteocalcin level tended to parallel those for calcium, whereas levels of the pyridinium crosslinks tended to increase with age (Chapter 4). Neither group of markers were sufficiently sensitive to detect differences in bone tumover between lambs of similar age but growing at different rates (Chapter 5). At slaughter the faster growing lambs had heavier though less well mineralised bones but showed no change in the ratio of calcium/osteocalcin. The ratio of calcium/osteocalcin in bone was also unchanged in lambs fed diets deficient in phosphorus (Chapter 6) despite an overall decline in mineral and in plasma and bone osteocalcin contents, which appeared to support the view that plasma and bone osteocalcin contents, which appeared to support the view thatosteocalcin may play a functional role in the mineralization process. However, against this, pigs fed diets deficient in both calcium and phosphorus showed no change in either plasma or bone osteocalcin levels despite marked reductions in bone calcium content (Chapter 7). This suggests that the amount of osteocalcin deposited in bone is not simply related to the amount of mineral present and so calls into question whether it has a direct role in the mineralization process. Chapter 8 describes an experiment in which adult sheep were treated with one of the new bisphosphonates (Ibandronate) and its effects on the metabolism of both pyridinoline and deoxypyridinoline were monitored. Given at rates which have been shown to be effective in reducing bone resorption in humans, this compound had little effect on the overall rates of excretion of these crosslinks but did alter the proportions excreted in free or peptide bound form. Another feature of this study was the high variability seen in the escretion of these markers in urine both between animals and between collection periods with coeficients of variation of 48 and 36 % for total pyridinoline and deoxypyridinoline and 62 and 50 % for their free fractions. Similar high variability has been reported by other workers and has clear implications in regard to the accuracy of the these markers.
650 $abone metabolism
650 $abones
650 $aosteocalcin
650 $aphysiology
650 $aswine
650 $aDiagnostico
650 $aFisiologia
650 $aOsso
650 $aOvino
650 $aSuíno
653 $aBiochemical markers
653 $aBone markers
653 $aDeoxypiridinoline
653 $aDiagnosis
653 $aDioxidipirionolina
653 $aMarcador bioquimico
653 $aMarcador osseo
653 $aMetabolismo osseo
653 $aOsteocalcina
653 $aOvine
653 $aPiridinolina
653 $aPyridinoline
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Embrapa Gado de Corte (CNPGC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
31/03/2009 |
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Data da última atualização: |
30/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
LEÓN, M. G.; BECERRA, C. H.; FREITAS-ASTÚA, J.; SALAROLI, R. B.; KITAJIMA, E. W. |
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Afiliação: |
M. G. León, CORPOICA; C. H. Becerra, ICA; Juliana Freitas-Ástua, CNPMF; R. B. Salaroli, ESALQ; Elliot Watanabe Kitajima, ESALQ. |
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Título: |
Natural infection of Swinglea glutinosa by the Citrus leprosis virus cytoplasmic type (CiLV-C) in Colombia. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Plant Disease, v.92, n.9, p.1364, 2008. |
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Idioma: |
Inglês |
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Notas: |
DOI: 10.1094/PDIS-92-9-1364C |
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Conteúdo: |
Swinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is originally from southeast Asia but which is now grown worldwide. In Colombia, it is used as an ornamental and principally as a living fence around rural properties and farms in several regions of the country. Citrus leprosis virus cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of the potential for country-wide infection of citrus, some measures are being taken to avoid CiLV-C spread to other regions of Colombia. Further surveys made from June to December 2005 to evaluate the extent of the spread of CiLV-C in the Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, similar to those caused by CiLV-C on sweet oranges leaves. These plants were found near citrus orchards in the municipalities of Guamal and in some urban areas of Villavicencio City in the Meta Department. The possibility that these symptoms were caused by CiLV-C was investigated soon after sample collection by the same procedures as described previously for sweet orange (2). In the leaf lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic viroplasm were found with electron microscopy. Total RNA extracted from symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers (Fwd. 5'GATACGGGACGCATAACA-3'/Rev. 5'-TTCTGGCTCAACATCTGG-3') that specifically amplify a region within the CiLV-C putative methyltransferase gene and this yielded a single fragment of the expected 402 bp (3). Analysis of the consensus sequence derived from 20 RT-PCR products (GenBank Accession No. EU689106) showed 96% nucleotide and 92% amino acid sequence identity to the sequence of a Brazilian CiLV-C isolate (GenBank Accession Nos. DQ352194.1 and YP_654565.1), respectively. Recently, published work described mite transmission of CiLV-C to some nonrutaceous plants (1), but to our knowledge, this is the first report of a nonCitrus rutaceous plant naturally infected by CiLV-C. Mites found in citrus orchards and previously identified as Brevipalpus phoenicis (Geijskes) (2), which are likely the most important vector of CiLV-C in citrus in Colombia, were observed feeding on healthy and symptomatic S. glutinosa, indicating that S. glutinosa is a host for B. phoenicis. Because the use of S. glutinosa as a living fence or hedge is a common practice in Colombia, CiLV-C-infected S. glutinosa plants may play a role in the epidemiology of leprosis in commercial citrus by serving as an inoculum source for this lethal virus. MenosSwinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is originally from southeast Asia but which is now grown worldwide. In Colombia, it is used as an ornamental and principally as a living fence around rural properties and farms in several regions of the country. Citrus leprosis virus cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of the potential for country-wide infection of citrus, some measures are being taken to avoid CiLV-C spread to other regions of Colombia. Further surveys made from June to December 2005 to evaluate the extent of the spread of CiLV-C in the Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, similar to those caused by CiLV-C on sweet oranges leaves. These plants were found near citrus orchards in the municipalities of Guamal and in some urban areas of Villavicencio City in the Meta Department. The possibility that these symptoms were caused by CiLV-C was investigated soon after sample collection by the same procedures as described previously for sweet orange (2). In the leaf lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic viroplasm were found with electron microscopy. Total RNA extracted from symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers (Fwd. 5... Mostrar Tudo |
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Thesagro: |
Ácaro; Doença de Planta; Leprose; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03395naa a2200229 a 4500
001 1655641
005 2023-06-30
008 2008 bl uuuu u00u1 u #d
100 1 $aLEÓN, M. G.
245 $aNatural infection of Swinglea glutinosa by the Citrus leprosis virus cytoplasmic type (CiLV-C) in Colombia.$h[electronic resource]
260 $c2008
500 $aDOI: 10.1094/PDIS-92-9-1364C
520 $aSwinglea glutinosa (Blanco) Merr., a perennial plant in the family Rutaceae, is originally from southeast Asia but which is now grown worldwide. In Colombia, it is used as an ornamental and principally as a living fence around rural properties and farms in several regions of the country. Citrus leprosis virus cytoplasmic type (CiLV-C) was recently detected in orange groves of the Colombian Piedmont eastern plains, an area known as the Llanos Orientales (2). Because of the potential for country-wide infection of citrus, some measures are being taken to avoid CiLV-C spread to other regions of Colombia. Further surveys made from June to December 2005 to evaluate the extent of the spread of CiLV-C in the Llanos Orientales revealed some plants in S. glutinosa hedges surrounding citrus orchards exhibiting chlorotic spots and ringspots of varied size on the leaves, similar to those caused by CiLV-C on sweet oranges leaves. These plants were found near citrus orchards in the municipalities of Guamal and in some urban areas of Villavicencio City in the Meta Department. The possibility that these symptoms were caused by CiLV-C was investigated soon after sample collection by the same procedures as described previously for sweet orange (2). In the leaf lesions of S. glutinosa, typical bacilliform particles and dense cytoplasmic viroplasm were found with electron microscopy. Total RNA extracted from symptomatic leaves was subjected to reverse transcription-PCR (RT) using primers (Fwd. 5'GATACGGGACGCATAACA-3'/Rev. 5'-TTCTGGCTCAACATCTGG-3') that specifically amplify a region within the CiLV-C putative methyltransferase gene and this yielded a single fragment of the expected 402 bp (3). Analysis of the consensus sequence derived from 20 RT-PCR products (GenBank Accession No. EU689106) showed 96% nucleotide and 92% amino acid sequence identity to the sequence of a Brazilian CiLV-C isolate (GenBank Accession Nos. DQ352194.1 and YP_654565.1), respectively. Recently, published work described mite transmission of CiLV-C to some nonrutaceous plants (1), but to our knowledge, this is the first report of a nonCitrus rutaceous plant naturally infected by CiLV-C. Mites found in citrus orchards and previously identified as Brevipalpus phoenicis (Geijskes) (2), which are likely the most important vector of CiLV-C in citrus in Colombia, were observed feeding on healthy and symptomatic S. glutinosa, indicating that S. glutinosa is a host for B. phoenicis. Because the use of S. glutinosa as a living fence or hedge is a common practice in Colombia, CiLV-C-infected S. glutinosa plants may play a role in the epidemiology of leprosis in commercial citrus by serving as an inoculum source for this lethal virus.
650 $aÁcaro
650 $aDoença de Planta
650 $aLeprose
650 $aVírus
700 1 $aBECERRA, C. H.
700 1 $aFREITAS-ASTÚA, J.
700 1 $aSALAROLI, R. B.
700 1 $aKITAJIMA, E. W.
773 $tPlant Disease$gv.92, n.9, p.1364, 2008.
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